Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line)
The culture of COS-7 cells displayed in the digital image above was immunofluorescently labeled with primary anti-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy3, targeting intracellular microtubules. The culture was also stained for nuclear DNA with the ultraviolet-absorbing probe DAPI and for filamentous actin with Alexa Fluor 488 conjugated to phalloidin. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.