Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line)

The adherent COS-7 fibroblast cell culture presented in the digital image above was labeled for the cytoskeletal filamentous actin and intracellular mitochondrial networks with Alexa Fluor 546 conjugated to phalloidin and MitoTracker Deep Red 633, respectively. Nuclei present in the cells were counterstained with SYTOX Green. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of MitoTracker Deep Red 633, which was pseudocolored yellow.