Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line)

Presented in the digital image above is an adherent culture of COS-7 fibroblasts that was labeled with Oregon Green 488 conjugated to soybean agglutinin, which has saccharide binding sites that recognize and bind terminal alpha- and beta-N-acetylgalactosamine and galactopyranosyl oligosaccharides. The cells were also labeled with Alexa Fluor 568 conjugated to phalloidin and DAPI, targeting the cytoskeletal F-actin network and nuclear DNA, respectively. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.