Transformed (Simian Virus 40) African Green Monkey Kidney Fibroblast Cells (COS-7 Line)
The culture of transformed monkey kidney cells (COS-7 line) that is presented in the digital image above was labeled with SYTOX Orange and Alexa Fluor 488 conjugated to phalloidin, which target DNA and F-actin, respectively. In addition, the mitochondria were stained with MitoTracker Deep Red 633. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles with the exception of MitoTracker Deep Red 633, which was pseudocolored blue.