Wheat Grain Thin Section

DAPI-FITC-TRITC Bandpass Emission (Triple Band Excitation) Set

Autofluorescence emission intensity from a thin section of wheat grain (Triticum aestivum) tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll. In the blue and green excitation regions, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 450 and 550 nanometers. Other endogenous fluorophores are excited by ultraviolet light with this filter combination. For the wheat grain tissue illustrated above, note the presence of autofluorescence emission intensity in the blue, green, and red spectral regions, which is characteristic of the Nikon DAPI-FITC-TRITC fluorescence filter combination.