negative phase contrast microscopy

Phase contrast microscopy where the phase plate retards the un-diffracted light relative to the diffracted light by a quarter wavelength (λ/4). Since diffracted light is retarded by ~λ/4 through interaction with the object, the phase difference is eliminated and constructive interference occurs.

Synonyms: negative phase contrast

See also: phase , phase object , phase plate , phase difference , phase contrast microscopy , interference , positive phase contrast microscopy

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Positive and Negative Phase Contrast

This interactive tutorial explores relationships between the surround (S), diffracted (D), and resulting particle (P) waves in brightfield as well as positive and negative phase contrast microscopy.