phase plate

Used in phase contrast microscopy, a phase plate is (usually) placed in the objective lens back focal plane and features a circular modulator conjugate to the circular illumination mask of the phase annulus. It both attenuates light passing through it and advances or retards its phase by a quarter wavelength.

See also: phase annulus , phase contrast microscopy , positive phase contrast microscopy , negative phase contrast microscopy , phase contrast condenser

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Introduction to Phase Contrast Microscopy

A mechanism to translate variations in phase into corresponding changes in amplitude.