non-descanned detection (NDD)

A strategy for detecting emitted light immediately near a sample following excitation. It is preferred for single-point detection with multiphoton microscopy because it minimizes the number of optical components between the sample and detector, maximizing collection efficiency.

See also: multiphoton microscopy , photomultiplier tube

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Multiphoton Microscopy

Mode-locked pulsed lasers are used for deep tissue imaging and optical sectioning.