non-linear optics (NLO)

A subfield focusing on non-linear behavior of light in a non-linear system. For example, two-photon absorption follows a non-linear curve dependent on excitation light intensity.

See also: multiphoton microscopy, two-photon excitation, three-photon excitation, second harmonic generation microscopy, third harmonic generation microscopy, Raman scattering

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Multiphoton Microscopy

Mode-locked pulsed lasers are used for deep tissue imaging and optical sectioning.