light sheet fluorescence microscopy (LSFM)

A technique in which a thin sheet of light is generated perpendicular to the optical path of the microscope to illuminate the sample, minimizing exposure outside of the focal plane and thus reducing photobleaching and phototoxicity. Can be scanned to observe large sample volumes quickly.

Synonyms: selective plane illumination microscopy , ultramicroscopy

See also: confocal theta microscopy , optical section , multi-view fusion , water dipping objective , Bessel beam , Airy beam

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Light Sheet Fluorescence Microscopy

Planar illumination techniques for fast 3D imaging of larger specimens with minimal light dosage.