The resolving power of a microscope is the most important feature of the optical system.
Basic equipment and techniques necessary for observing specimens in fluorescence.
Using crossed polarized illumination to examine birefringent materials.
A mechanism to translate variations in phase into corresponding changes in amplitude.
The ability of a microscope objective to gather light and resolve fine specimen detail.
The ratio of the speed of light in a vacuum to that in the imaging medium of a microscope.
Fundamentals of the axial or longitudinal properties of microscope objectives.
A discussion of point scanning and pinhole detection using photomultipliers.
Defined as double refraction of light in a transparent, molecularly ordered material.
TIRF restricts the excitation and detection of fluorophores to a thin region of the specimen.
The nomenclature and abbreviations inscribed on the objective protective barrel.
Genetically-encoded fluorescent probes that are revolutionizing live-cell imaging.
Mode-locked pulsed lasers are used for deep tissue imaging and optical sectioning.
Limitations on optical microscope resolution imposed by physical laws.
The eyepiece field diaphragm determines the diameter (size) of the microscope viewfield.
These microscopes feature a parallel optical path between the objective and the tube lens.
The distance between the objective front lens or the nosepiece and the specimen.
Fundamental properties of CCDs, including pixels, readout, noise, and timing.
The Nikon yellow fluorescent protein fluorescence filter category comprises a single high-performance balanced combination, which effectively extends the fluorescent protein detection capabilities afforded by the three green fluorescent protein (GFP) filter sets to the longer wavelength enhanced yellow chromatic variants of GFP (YFP and EYFP). The YFP HYQ filter combination...
Discussion of birefringence, Brewster's angle, and various forms of polarized light.