Popular Tutorials

  1. Stochastic Optical Reconstruction Microscopy (STORM) Imaging

    Review the basic steps involved in acquiring a STORM super-resolution image.

  2. Spatial Resolution in Digital Imaging

    Spatial resolution refers to the number of pixels utilized in construction of the image.

  3. Laser Scanning Confocal Microscopy

    A virtual microscope tutorial featuring a wide variety of specimens.

  4. Microscope Alignment for Köhler Illumination

    Learn how to adjust a microscope to examine specimens in Köhler illumination.

  5. Numerical Aperture and Image Resolution

    Explore how objective numerical aperture size influences Airy disk properties.

  6. Objective Working Distance

    Examine the range of working distance values available in modern Nikon CFI60 objectives.

  7. Matching Camera to Microscope Resolution

    Vary numerical aperture, magnification, and video coupler size to match camera resolution.

  8. Comparison of Phase Contrast and DIC Microscopy

    Examples of the same specimen viewed in either phase contrast or DIC.

  9. Field of View Diameter

    Exploring the effect of varying the field of view size on the viewable specimen area.

  10. CCD Signal-To-Noise Ratio

    Signal-to-noise represents the ratio of the measured light signal to the combined noise.

  1. Choosing Filter Combinations for Fluorescent Proteins

    Identification of critical filter parameters for imaging fluorescent proteins.

  2. Depth of Field Calculator

    Calculate the depth of field for popular objectives with this interactive tutorial.

  3. Immersion Oil and Refractive Index

    The refractive index is critical in determining the working numerical aperture of an objective.

  4. Eyepiece Reticle Calibration

    Explores calibration of various eyepiece reticles using a stage micrometer and demonstrates how the reticle can then be employed to determine linear specimen dimensions.

  5. Phase Contrast Microscope Alignment

    Learn how to align a phase contrast microscope and examine variations in specimen appearance through the eyepieces (at different magnifications) when the condenser annulus is shifted into and out of alignment with the phase plate in the objective.

  6. Astigmatism

    An off-axis aberration manifested as a point appearing to be a line or ellipse.

  7. Adjustment of Objective Correction Collars

    Learn how to adjust an objective correction collar to minimize spherical aberration.

  8. Focus and Alignment of Mercury and Xenon Arc Lamps

    Explore alignment and focusing of the arc lamp in a mercury or xenon burner, which simulates how the lamp is adjusted in a real microscope.

  9. The Nikon Perfect Focus System (PFS)

    Examine how the Nikon PFS system operates to maintain stable focus in live-cell imaging.

  10. Positive and Negative Phase Contrast

    This interactive tutorial explores relationships between the surround (S), diffracted (D), and resulting particle (P) waves in brightfield as well as positive and negative phase contrast microscopy.