Popular Tutorials
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Spatial Resolution in Digital Imaging
Spatial resolution refers to the number of pixels utilized in construction of the image.
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Stochastic Optical Reconstruction Microscopy (STORM) Imaging
Review the basic steps involved in acquiring a STORM super-resolution image.
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Numerical Aperture and Image Resolution
Explore how objective numerical aperture size influences Airy disk properties.
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Matching Camera to Microscope Resolution
Vary numerical aperture, magnification, and video coupler size to match camera resolution.
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Comparison of Phase Contrast and DIC Microscopy
Examples of the same specimen viewed in either phase contrast or DIC.
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Microscope Alignment for Köhler Illumination
Learn how to adjust a microscope to examine specimens in Köhler illumination.
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Objective Working Distance
Examine the range of working distance values available in modern Nikon CFI60 objectives.
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Field of View Diameter
Exploring the effect of varying the field of view size on the viewable specimen area.
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Laser Scanning Confocal Microscopy
A virtual microscope tutorial featuring a wide variety of specimens.
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Immersion Oil and Refractive Index
The refractive index is critical in determining the working numerical aperture of an objective.
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CCD Signal-To-Noise Ratio
Signal-to-noise represents the ratio of the measured light signal to the combined noise.
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Birefringence in Calcite Crystals
Examine how crystals of the mineral calcite demonstrate birefringence.
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Birefringent Crystals in Polarized Light
Explore how birefringent anisotropic crystals interact with polarized light in an optical microscope as the circular stage is rotated through 360 degrees.
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Choosing Filter Combinations for Fluorescent Proteins
Identification of critical filter parameters for imaging fluorescent proteins.
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Depth of Field Calculator
Calculate the depth of field for popular objectives with this interactive tutorial.
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Tube Lens Focal Length
Infinity optical systems feature tube lengths between 200 and 250 millimeters.
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Chromatic Aberration
Explore axial and lateral chromatic aberrations seen in an optical microscope with this interactive tutorial.
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Numerical Aperture Light Cones
Numerical aperture is a measure of the highly diffracted light rays captured by the objective.
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Positive and Negative Phase Contrast
This interactive tutorial explores relationships between the surround (S), diffracted (D), and resulting particle (P) waves in brightfield as well as positive and negative phase contrast microscopy.